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2003•Journal of Forensic MedicineRequires access

Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats

Xiaohu Xu

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Abstract

Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R.

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What this paper is about

Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R.

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Available abstract

Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R.

Key concepts: Ischemia, Messenger RNA, Staining, Reperfusion injury, Myocardial ischemia, Medicine, Myocyte, Immunohistochemistry

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Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats — Research Paper | ScholarLens