The accuracy evaluation of nucleated red blood cell count determined by SYSMEX XN-1000 automatic hematology analyzer

Objective To investigate the accuracy of nucleated red blood cell( NRBC) count determined by SYSMEX XN-1000 automatic hematology analyzer( XN-1000). Methods A total of 66 peripheral blood samples were collected and determined for NRBC by XN-1000,and the results of NRBC% were compared with those by flow cytometry( FCM) and manual microscopy for the same sample. The manual microscopy was as golden standard. Results There was a good correlation for NRBC between XN-1000 and microscopy( r = 0. 927),but the correlation was lower than that between FCM and manual microscopy( r = 0. 956). The correlation between XN-1000 and FCM was the best( r = 0. 994). The results of XN-1000 and FCM with manual microscopy had no statistical significance( P 0. 05). XN-1000 for NRBC% had a better specificity( 95. 7%) than FCM. Conclusions XN-1000 automatic hematology analyzer has good performance for detecting NRBC in peripheral blood rapidly,accurately and effectively.