Effect of rosiglitazone on leptin and TNF-α of the metabolic syndrome rats

OBJECTIVE To investigate the relation between insulin resistance and leptin or TNF-α by observed the change of leptin and TNFα in metabolic syndrome rats,To study the effect of rosiglitazone on leptin and TNF-α of the metabolic syndrome rats by observing the change of leptin and TNF-α.METHODS SD rats were divided into 2 groups randomly:the control group,the fat group.After 12 weeks the blood glucose,blood;lipid and serium insulin,were measured,Homa IR was used to estimate the insulin resistance.Leptin in adipocyte was detected by Immunohistological(ABC) method.Than the successed models of the fat group were divided into 2 group;the fat group and the rosiglitazone-treated fat group,after 4 weeks,observed the change of leptin and TNF-α.RESULTS After 12 weeks,the blood glucose,blood;lipid,serium insulin,leptin and TNF-α in the fat group were more high than that in control group.After 4 weeks,leptin and TNF-α of the rosiglitazone-treated fat group were decreased obviously[(0.41±0.23)∶(0.19±0.49),P0.05][(356.8±87.0)∶(196.9±32.5),P0.01].CONCLUSION High fat and high sucrose diet induce the model of rat of metabolic syndrome.There are close relationship between insulin resistance and serum leptin and TNF-α.Function of rosiglitazone is implemented by effection on leptin and TNF-α.