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2010•Journal of University of South ChinaRequires access

TRAIL in Induction of Apoptosis on Human Colon Cancer SW480 Cell Line

Yujuan Zhou

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Abstract

Objective To investigate the effect of TRAIL induced apoptosis in Human colon cancer SW480 cell line.Methods The experiment was divided into two groups: treated group with rmhTRAIL and negative control group.SW480 cells proliferation was measured by MTT assay.The apoptosis rate was analyzed by flow cytometry(FCM).The expression of Bcl-2,Bax was detected by western blot. Results MTT showed that rmhTRAIL significantly inhibited SW480 cells proliferation with a dose-dependent manner(P0.05).When SW480 cells treated by rmhTRAIL,the FCM revealed that rmhTRAIL could markedly upgrade the apoptosis rate(P0.01),and the expression of Bcl-2 downregulated markedly(P0.05),while the expression of Bax upregulated significantly(P0.05).Conclusions rmhTRAIL could induce apoptosis in human colon cancer SW480 cell line,and the mechanism was related to downregulation expression of Bcl-2,upregulation expression of Bax.

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Objective To investigate the effect of TRAIL induced apoptosis in Human colon cancer SW480 cell line.Methods The experiment was divided into two groups: treated group with rmhTRAIL and negative control group.SW480 cells proliferation was measured by MTT assay.The apoptosis rate was analyzed by flow cytometry(FCM).The expression of Bcl-2,Bax was detected by western blot. Results MTT showed that rmhTRAIL significantly inhibited SW480 cells proliferation with a dose-dependent manner(P0.05).When SW480 cells treated by rmhTRAIL,the FCM revealed that rmhTRAIL could markedly upgrade the apoptosis rate(P0.01),and the expression of Bcl-2 downregulated markedly(P0.05),while the expression of Bax upregulated significantly(P0.05).Conclusions rmhTRAIL could induce apoptosis in human colon cancer SW480 cell line,and the mechanism was related to downregulation expression of Bcl-2,upregulation expression of Bax.

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Available abstract

Objective To investigate the effect of TRAIL induced apoptosis in Human colon cancer SW480 cell line.Methods The experiment was divided into two groups: treated group with rmhTRAIL and negative control group.SW480 cells proliferation was measured by MTT assay.The apoptosis rate was analyzed by flow cytometry(FCM).The expression of Bcl-2,Bax was detected by western blot. Results MTT showed that rmhTRAIL significantly inhibited SW480 cells proliferation with a dose-dependent manner(P0.05).When SW480 cells treated by rmhTRAIL,the FCM revealed that rmhTRAIL could markedly upgrade the apoptosis rate(P0.01),and the expression of Bcl-2 downregulated markedly(P0.05),while the expression of Bax upregulated significantly(P0.05).Conclusions rmhTRAIL could induce apoptosis in human colon cancer SW480 cell line,and the mechanism was related to downregulation expression of Bcl-2,upregulation expression of Bax.

Key concepts: Apoptosis, Downregulation and upregulation, Flow cytometry, Cell culture, Western blot, MTT assay, Cell growth, Cell

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