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2014•Guizhou nongye kexueRequires access

ISSR Analysis of Genetic Diversity of Miao Medicine Malan Grass in Guizhou

Xiong Canxi

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Abstract

To provide a reference for utilizing Miao medicine Malan grass germplasm,the genetic diversity of 10representational populations of Malan grass including 100individuals were investigated by ISSR marker technique,and POPGEN32was used in data analysis and UPGMA dendrogram was drawn. Results:A total of 66bands were produced in 9individuals,of which 46bands were common in the 10 population.The value of the polymorphic bands(PPB)was 79.70%,Nei's genetic diversity index(He) was 0.211 7,Shannon's inform ation index(I)was 0.325 8,the genetic differentiation index(Gst)was 0.714 2,The genetic similarity among populations coefficient was 0.083 1~0.940 5,with an average of 0.596 1;Groups cluster analysis based on the UPGMA method indicated that the 10populations of Malan grass were divided into three groups.

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What this paper is about

To provide a reference for utilizing Miao medicine Malan grass germplasm,the genetic diversity of 10representational populations of Malan grass including 100individuals were investigated by ISSR marker technique,and POPGEN32was used in data analysis and UPGMA dendrogram was drawn. Results:A total of 66bands were produced in 9individuals,of which 46bands were common in the 10 population.The value of the polymorphic bands(PPB)was 79.70%,Nei's genetic diversity index(He) was 0.211 7,Shannon's inform ation index(I)was 0.325 8,the genetic differentiation index(Gst)was 0.714 2,The genetic similarity among populations coefficient was 0.083 1~0.940 5,with an average of 0.596 1;Groups cluster analysis based on the UPGMA method indicated that the 10populations of Malan grass were divided into three groups.

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Available abstract

To provide a reference for utilizing Miao medicine Malan grass germplasm,the genetic diversity of 10representational populations of Malan grass including 100individuals were investigated by ISSR marker technique,and POPGEN32was used in data analysis and UPGMA dendrogram was drawn. Results:A total of 66bands were produced in 9individuals,of which 46bands were common in the 10 population.The value of the polymorphic bands(PPB)was 79.70%,Nei's genetic diversity index(He) was 0.211 7,Shannon's inform ation index(I)was 0.325 8,the genetic differentiation index(Gst)was 0.714 2,The genetic similarity among populations coefficient was 0.083 1~0.940 5,with an average of 0.596 1;Groups cluster analysis based on the UPGMA method indicated that the 10populations of Malan grass were divided into three groups.

Key concepts: UPGMA, Genetic diversity, Germplasm, Dendrogram, Diversity index, Genetic similarity, Biology, Population

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