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ISSR Analysis of Genetic Diversity of Asparagus cochinchinensis

Min Wang

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Abstract

The genetic diversity of 67 individuals from 19 populati-ons was analyzed by ISSR.The results showed that 13 primers were selected to produce highly reproducible ISSR bands,92 showed polymorphism among 125 amplified bands and the percentage of polymorphic bands reached 73%,ISSR marker of different populations was found.Nei' s gene diversity index(H) was 0.192,Shannon's information index was 0.30,genetic differentiation index was 0.8206.The Clustering tree showed the populations of Qinghai and Yunnan province,5 populations of Guizhou province were a branch,others populations were another branch.Asparagus had a high genetic diversity and the majority of genetic variation occurred among populations.The ISSR marker could be used for analysis of genetic diversity and identification of genetic variation of Asparagus cochinchinensis.

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What this paper is about

The genetic diversity of 67 individuals from 19 populati-ons was analyzed by ISSR.The results showed that 13 primers were selected to produce highly reproducible ISSR bands,92 showed polymorphism among 125 amplified bands and the percentage of polymorphic bands reached 73%,ISSR marker of different populations was found.Nei' s gene diversity index(H) was 0.192,Shannon's information index was 0.30,genetic differentiation index was 0.8206.The Clustering tree showed the populations of Qinghai and Yunnan province,5 populations of Guizhou province were a branch,others populations were another branch.Asparagus had a high genetic diversity and the majority of genetic variation occurred among populations.The ISSR marker could be used for analysis of genetic diversity and identification of genetic variation of Asparagus cochinchinensis.

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Available abstract

The genetic diversity of 67 individuals from 19 populati-ons was analyzed by ISSR.The results showed that 13 primers were selected to produce highly reproducible ISSR bands,92 showed polymorphism among 125 amplified bands and the percentage of polymorphic bands reached 73%,ISSR marker of different populations was found.Nei' s gene diversity index(H) was 0.192,Shannon's information index was 0.30,genetic differentiation index was 0.8206.The Clustering tree showed the populations of Qinghai and Yunnan province,5 populations of Guizhou province were a branch,others populations were another branch.Asparagus had a high genetic diversity and the majority of genetic variation occurred among populations.The ISSR marker could be used for analysis of genetic diversity and identification of genetic variation of Asparagus cochinchinensis.

Key concepts: Genetic diversity, Asparagus, Biology, Diversity index, Genetic variation, Genetic distance, Botany, Microsatellite

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