ISSR analysis on genetic diversity in Phyllanthus urinaria

Objective To detect the genetic diversity of 30 populations of Phyllanthus urinaria from some provinces of China.Methods ISSR was used to analyze the genetic diversity of 30 populations of P.urinaria from Chongqing,Shaanxi,Henan,Yunnan,Guangxi,Guangdong,Zhejiang,Guizhou,Fujian,and Hainan Provinces of China.Results Twenty four primers selected from 60 ISSR primers were used for amplification and a total of 264 DNA bands were obtained,including 245 polymorphic bands and 19 common bands.At species level,the average percentage of polymorphic bands(PPB) was 92.80%.The range of the genetic similarity(GS) value was 0.579 5-0.916 7,with a higher genetic diversity.Cluster analysis showed that the P.urinaria population was roughly divided into three branches: inland branch,coastal branch,and transition branch which located in Yunnan Province;Individual populations presented obvious specificity.Conclusion The genetic diversity of germplasm resources in P.urinaria is higher.