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2006•Chinese Journal of Laboratory DiagnosisRequires access

Comparison of three simple methods for genomic DNA extraction

Xiangdong Tu

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Abstract

Objective To choose a fast,easy,hold ture and low cost for genomic DNA extraction whole human blood.Methods Rapid phenol-chloroform method,salting out method and potassium iodide method were used to extract genomic DNA from human blood.Results Both Rapid phenol and postassium iodide methods all would give high quality of DNA,but the yield and purity at DNA prepared with Salting out method was comparatively low.Conclusion improve potassium iodide methods wes recommended for routine DNA extraction from whole human blood.

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Objective To choose a fast,easy,hold ture and low cost for genomic DNA extraction whole human blood.Methods Rapid phenol-chloroform method,salting out method and potassium iodide method were used to extract genomic DNA from human blood.Results Both Rapid phenol and postassium iodide methods all would give high quality of DNA,but the yield and purity at DNA prepared with Salting out method was comparatively low.Conclusion improve potassium iodide methods wes recommended for routine DNA extraction from whole human blood.

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Available abstract

Objective To choose a fast,easy,hold ture and low cost for genomic DNA extraction whole human blood.Methods Rapid phenol-chloroform method,salting out method and potassium iodide method were used to extract genomic DNA from human blood.Results Both Rapid phenol and postassium iodide methods all would give high quality of DNA,but the yield and purity at DNA prepared with Salting out method was comparatively low.Conclusion improve potassium iodide methods wes recommended for routine DNA extraction from whole human blood.

Key concepts: Salting out, genomic DNA, DNA, Extraction (chemistry), DNA extraction, Chromatography, Iodine, Iodide

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