Drug sensitizing effect and related mechanisms of Fas gene transduction on human drug-resis-tant gastric cancer cell SGC7901/VCR

Aim To observe the drug sensitizing effect and related mechanisms of Fas gene transduction on human drugresistant gastric cancer cell SGC7901/VCR. Methods The cell cycle alteration, the sensitivity of gastric cancer cells to several antitumor drugs and expression of Pgp and Topo II in gastric cancer cells were detected by FCM, MTT assay and immunocytochemical staining,respectively. Results By comparing with SGC7901 and pBKSGC7901/VCR,FasSGC7901/VCR cells decrease in the G2 stage and increase in the S stage, and obviously apoptotic peak arisen. Increased sensitivity of FasSGC7901/VCR to DDP, MMC and 5Fu;Topo II expression among SGC7901, pBKSGC7901/VCR and FasSGC7901/VCR had no obvious differences;Pgp expression level in pBKSGC7901/VCR was lower than that in SGC7901. Conclusion Fas gene transduction could reverse the multidrug resistance(MDR)of human drugresistant gastric cancer cell SGC7901/VCR, for which the higher sensitivity to apoptosis and decreased expression of Pgp may be responsible.