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2003•Journal of Comprehensive StomatologyRequires access

The Effect of TGF-β1 on the Proliferation and Cell Cycle of MDPC-23 Cells.

Shouliang Zhao

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Abstract

Objective: To evaluate the effects of transforming growth factor-β1 (TGF-β1) on the proliferation and cell cycle of odontoblast cell line MDPC-23.Methods:Cell culture, MTT colorimetric assay and flow cytometry (FCM) were used. Results: TGF-β1 significantly inhibited the proliferation of MDPC-23 cells in a time-dependent manner, but without dose-dependent manner. The results of FCM analysis showed that S% and (S+G 2M)of cell cycle was decreased, while G 1% was increased, when the cells were stimulated by TGF-β1. Conclusions: TGF-β1 inhibits the proliferation and DNA synthesis of odontoblast cell line MDPC-23.

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What this paper is about

Objective: To evaluate the effects of transforming growth factor-β1 (TGF-β1) on the proliferation and cell cycle of odontoblast cell line MDPC-23.Methods:Cell culture, MTT colorimetric assay and flow cytometry (FCM) were used. Results: TGF-β1 significantly inhibited the proliferation of MDPC-23 cells in a time-dependent manner, but without dose-dependent manner. The results of FCM analysis showed that S% and (S+G 2M)of cell cycle was decreased, while G 1% was increased, when the cells were stimulated by TGF-β1. Conclusions: TGF-β1 inhibits the proliferation and DNA synthesis of odontoblast cell line MDPC-23.

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Available abstract

Objective: To evaluate the effects of transforming growth factor-β1 (TGF-β1) on the proliferation and cell cycle of odontoblast cell line MDPC-23.Methods:Cell culture, MTT colorimetric assay and flow cytometry (FCM) were used. Results: TGF-β1 significantly inhibited the proliferation of MDPC-23 cells in a time-dependent manner, but without dose-dependent manner. The results of FCM analysis showed that S% and (S+G 2M)of cell cycle was decreased, while G 1% was increased, when the cells were stimulated by TGF-β1. Conclusions: TGF-β1 inhibits the proliferation and DNA synthesis of odontoblast cell line MDPC-23.

Key concepts: Odontoblast, Cell cycle, Cell growth, Flow cytometry, Chemistry, Cell culture, Transforming growth factor, MTT assay

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