Establishment of Initial Culture of Cerasus tianschanica Pojark

Taking the stem of Cerasus tianschanica Pojark which was endangered plant as material,the effects of different sterilization and different sterilization time on the explants of Cerasus tianschanica Pojark were studied,and the influence of different basic medias and different growth regulator composite concentrations on axillary proliferation were investigated in order to establish the initial culture system of Cerasus tianschanica Pojark.The results showed that the best sterilization of the explants was Alcohol 20 s+HgCl212 min and the germination rate of axillary bud in MS basic media was higher than B5 basic media.The initial culture system of Cerasus tianschanica Pojark was MS+1.5 mg/L 6-BA+0.1 mg/L IBA+30 g/L Sucrose+6 g/L Agar powder.