Relationship between HPV high-risk genotypes and cervical lesions

Objective To analyze the distribution difference of human papilloma virus(HPV)high-risk genotypes in different cervical lesions and to study the value of HPV genotype test in cervical screening.Methods Cervical exfoliative cells were collected from the people for cervical cancer screening in our hospital from July 2007 to August 2012.Then HPV genotyping test and liquid based cytology test were taken.Colposcopy and cervical biopsy were performed in patients whose HPV high-risk genotype was positive and liquid based cytology results were greater than or equal to atypicai squamous cells of unknown significance(ASC-US).Eventually cervical lesions were found in 348 patients,including cervical cancer and cervical intraepithelial neoplasia confirmed by pathology.Taking to the pathological diagnosis of cervical intraepithelial neoplasia(CIN)Ⅱ+as critical endpoint,we analyzed the relationship between HPV genotypes and cervical lesions.Results Among of 10838 cases,positive high-risk genotypes appeared in 1712 cases,accounting for 15.8%.The six most common high-risk genotypes were HPV 16,58,52,33,18 and 31.There were significant differences in the detection rates of HPV 16,58,52,33 and 31 between different cervical lesions,especially HPV 16 (χ2 =26.784,P=0.000). In the patients with chronic cervicitis,the most common high risk genotypes were HPV 58 (χ2 =23.909,P=0.000),HPV 52 (χ2 =31.870,P=0.000),HPV 33 (χ2 =14.281,P=0.002) and HPV 31 (χ2 =12.839,P= 0.004).The incidence of canceration in the patients with HPV16 and HPV18infection was significantly higher than those with other genotype infections(P=0.000).In single infection,HPV16 had a higher pathogenic risk than other genotypes (χ2 =59.8,P=0.000).In multiple infection,HPV18 had a higher pathogenics risk than other genotypes(χ2 =28.3,P=0.000).And the patients with multiple infection including HPV18were much more than the patients with single infection of HPV 18 (OR=11.9,P=0.000).Conclusion HPV16 and HPV18 are the most genotypes for the development of cervical cancer.HPV genotyping test is recommended for cervical screening and follow up after surgery.