Isolation and identification of chemical constituents of n-butanol extracts from leaves of Camellia sinensis(L.) O.Ktze

Objective To investigate the chemical constituents of n-butanol extracts from the leaves of Camellia sinensis(L.)O.Ktze.Methods The compounds were isolated by chromatography on silica gel,Sephadex LH-20 columns and the recrystallization methods.Chemical properties and NMR were employed for the structural identification.Results Ten compounds were obtained and elucidated as following:Kaempferol(1),quercetin(2),kaempferol-3-O-β-D-glucopyranoside(3),quercetin-3-O-β-D-glucopyranoside(4),kaempferol-3-O-rutinoside(5),rutin(6),quercetin-3-O-α-L-arabinofuranoside(7),n-butyl-β-D-fructopyranoside(8),methyl chlorogenate(9)and uracil(10),respectively.Conclusions Among them,compounds 8,9 and 10 are obtained from the Camellia genus for the first time,7 is obtained from the Camellia sinensis(L.)O.Ktze for the first time.