Antioxidant effects of acteoside on aged mice induced by D-galactose

Aim To study the effects of learning and memory and antioxidant effects of acteoside on aged mice induced by D-galactose(D-gal).Methods 72 Kunming mice were randomly divided into control group,aging model group,Huperzine-A group,and acteoside high-,middle-and low-doses groups.The control group mice were subcutaneously injected with normal saline in the nape of the neck for 90d.The mice in the rest of the groups were subcutaneously injected with D-gal(150 mg · kg-1·d-1) in the nape of neck.The mice in Huperzine-A group and acteoside groups were simultaneously treated by intragastric gavage from 71d to 90d.Their learning and memory abilities were detected with a Morris test.Then the level of SOD,glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) in serum and brain tissue of the mice were detected in each group.Results The acteoside improved the learning and memory abilities of the aging model group.Compared with control group,SOD,GSH-Px activity revealed a significant decrease and MAD content exhibited a significant increase in serum and brain tissue in aging model mice.Compared with aging model group,SOD and GSH-Px activity in serum and brain tissue exhibited a significant increase and MDA content decreased in acteoside treatment group.The antioxidant effects of Huperzine-A group resembled those of acteoside treatment group.Correlation analysis showed that learning and memory abilities were closely related to SOD and GSH-Px activity,and MDA content in mice serum and brain.Conclusion Acteoside improves the disability of learning and memeory induced by D-gal,and may have effect on suppressing the aging process through enhancing antioxidant activity,and decreasing MDA content.