2004•The Journal of Cervicodynia and LumbodyniaRequires access
The effect and its significance of recombinant human erythropoietin on cytokine-induced neutrophil chemoattractant gene expression after spinal cord injury in rats
Lianshun Jia
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Abstract
Objective To study the effect of recombinant human erythropoietin(rHuEPO) on cytokine-induced neutrophil chemoattractant(CINC-1) gene expression after spinal cord injury(SCI) in rats. Methods 102 SD rats were divided randomly into four groups. The modified Allen's model of spinal cord injury was established. The expression of CINC-1mRNA after SCI was measured by reverse transcription polymerase chain reaction(RT-PCR). Results The expression of CINC-1mRNA was detected in non-injured spinal cord ,quickly up-regulated after SCI, peaked at 6h post-injury. rHuEPO significantly reduced the expression of CINC-1mRNA in the injured cord in comparison to control animals receiving NS vehicle at 6?12h after SCI. Conclusion CINC-1 may play a negative role in the secondary SCI. rHuEPO reduces the expression of CINC-1mRNA in the injured spinal cord,has neuroprotective effect in the secondary SCI.