1406 example pregnancy intermediate stage amniotic cell culture and chromosomal karyotypes analysis

Objective:To analyze the chromosomal karyotypes of amniotic fluid cells in mid-gestation women with amniotic cell culture in accordance with the indications for prenatal diagnosis,explore the characteristics of fetal chromosomal,In order to constitute a basis for prenatal diagnosis. Methods:1406 cases pregnant women with the indications for prenatal diagnosis from Jan. 2010 to Dec. 2011 undergo amniocentesis by ultrasound monitoring,extract amniotic fluid 20 ml to culture,and performed the G-banding staining for karyotype analysis.Results:In 1406 example pregnant woman s amniotic fluid chromosome inspection result,the unusual nuclear 94 examples,including 33 cases with chromosomal number abnormality and 61 cases with chromosomal structural abnormality;the embryo chromosome rate of abnormality is 6.69%,which includes 15 cases of 21-trisomy,7 cases of 18-trisomy,5 cases of Turner Syndrome,2 cases of 47,XXX,6 cases of balanced translocation,27 cases of inversion,chromosome mosaicism 6 cases and other structural chromosomal abnormality in 26 cases. Conclusion:The amniotic cell culture and chromosome analysis plays an important part in prenatal diagnosis,may obviously reduce the chromosome disease patients birth rate.