Effects of chicken typeIIcollagen oral spraying on collagen-induced arthritis in mice

AIM To examine whether chicken typeⅡcollagen (CⅡ) oral spraying has the therapeutic effect on the collagen-induced arthritis (CIA) in mice and investigate its related immunological mechanisms. METHODS CIA mouse model was established. The effect of treatment with CⅡspraying in mice was measured by paw-swelling score. Splenocytes proliferation, activity of interleukin-1 (IL-1) produced by peritoneal macrophages(PM) and interleukin-2 (IL-2) by splenocytes were assayed by MTT method. RESULTS CⅡ oral spraying(5, 10, 20 μg·kg -1 ·d -1 ×10 d, d 25-35 after immunization) decreased the mean of arthritis scores in CIA mice. The effect of CⅡ spraying was comparable with that of ig CⅡ and prednisone. Meanwhile, CⅡ oral spraying (10, 20 μg·kg -1 ·d -1 ×10 d) suppressed the ConA-induced splenocyte proliferation and the activity of IL-1 and IL-2 in CIA mice. CONCLUSION CⅡoral spraying suppresses the polyarthritis of CIA mice, which suggests that CⅡoral spraying have therapeutic effect on CIA mice. Its mechanism maybe related to the modification of the abnormal immunological function of CIA mice.