Study on enzymatic hydrolysis technology of tilapia fishbone

The protein hydrolyte was prepared by hydrolysis tilapia fishbone with neutral protease.Taken hydrolysis degree as an optimum index,the effect of initial pH,hydrolysis temperature,solid-liquid ratio,and enzyme dosage and hydrolysis time on hydrolysis degree was studied.Based on single factor experiments,the optimum process condition was obtained by using Box-Behnken center-united design and surface response design.Under this condition,the effect of different pre-treatment methods including water bath,high pressure steam,ultrasonic wave,and microwave on hydrolysis degree were studied.The result showed that the optimum process condition was temperature 49℃,enzyme dosage 1 824U/g,hydrolysis time 6h and the hydrolysis degree of tilapia fishbone 33.7%.Most pre-treatment methods were harmful to subsequent hydrolysis of tilapia fishbone,expect 110℃ high pressure steam and ultrasound with the power of 180W.