Etiology of 288 cases with hand, foot and mouth disease

Objective To investigate the pathogen distribution of patients with hand, foot and mouth diseases(HFMD) hospitalized in Division of Pediatrics, Beijing Ditan Hospital in 2011. Methods Real-time fluorescence quantitative RT-PCR kits with three reagents: universal enterovirus primer, coxsackievirus A16(CoxA16) primers and enterovirus 71(EV71) primers were used to detect the samples, respectively. Results The enterovirus-positive rate was 51.74%(149/288). Among this, the positive rate of EV71, CoxA16 and other types of entervirus were 23.26%(67/288), 7.29%(21/288), 21.18%(61/288), respectively. And the study reveals that the positive rate of CoxA16 had significantly decreased, EV71 changed little, other types of entervirus significantly increased, during 2009-2011. Conclusions In extensively epidemic-period of 2011, the etiology of children with hand, foot and mouth disease hospitalized in our hospital was dominated alternately by EV71 and non-EV71, non-CoxA16 virus. The positive rate of non-EV71, non-CA16 virus in Beijing area has been persistently increased during 2009-2011.