Etiology of hand, foot and mouth disease in Beijing in 2010

Objective To investigate the pathogen distribution of patients with hand, foot and mouth diseases (HFMD) hospitalized in division of pediatrics, Beijing Ditan Hospital, Capital Medical University, in 2010. Methods Real-time fluorescence quantitative RT-PCR kits with three reagents: universal enterovirus primer, Coxsackievirus A16 (CA16) primer and enterovirus 71 (EV71) primer were used to detect the samples. Results The enterovirus-positive rate was 63.20% (213/337).Among this, the positive rates of enterovirus 71, Coxsackievirus A-16 and other types of enterovirus were 40.36% (136/337), 5.46%(19/337) and 17.21%(58/337), respectively. Conclusions In 2010, the etiology of children with hand, foot and mouth disease hospitalized in our hospital was dominated by enterovirus 71(EV71). The infection rates of enterovirus except enterovirus 71 and Coxsackievirus A16 are main pathogens. Non-CA16, non-EV71 virus infection were significant higher than Coxsackievirus A16 infection.