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Constructions of pEGFP-C2-p100-SN and pEGFP-C2-p100-TD recombinant plasmids

Yang Jie

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Abstract

Objective: To construct eukaryotic green fluorescence protein expressing recombinant plasmids, pEGFP-C2-p100-SN and pEGFP-C2-p100-TD, which contained human p100 Staphylococcal Nuclease (SN) domain and Tudor (TD) domain respectively. Methods: The p100-SN and p100-TD fragments were purified after PSG5-p100-SN and PSG5-p100-TD were digested by Eco RI and Bam HI. And then they were inserted into pEGFP-C2 fluorescent expressing vector respectively. Results: The fragments of p100-SN and p100-TD were detected in the products of the restriction enzyme digestion and PCR. Conclusion: The fluorescent expressing recombinant plasmids, which expressed p100-SN-GFP and p100-TD-GFP fusion proteins, are constructed successfully. The studies about the function of human p100 protein are put forward.

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What this paper is about

Objective: To construct eukaryotic green fluorescence protein expressing recombinant plasmids, pEGFP-C2-p100-SN and pEGFP-C2-p100-TD, which contained human p100 Staphylococcal Nuclease (SN) domain and Tudor (TD) domain respectively. Methods: The p100-SN and p100-TD fragments were purified after PSG5-p100-SN and PSG5-p100-TD were digested by Eco RI and Bam HI. And then they were inserted into pEGFP-C2 fluorescent expressing vector respectively. Results: The fragments of p100-SN and p100-TD were detected in the products of the restriction enzyme digestion and PCR. Conclusion: The fluorescent expressing recombinant plasmids, which expressed p100-SN-GFP and p100-TD-GFP fusion proteins, are constructed successfully. The studies about the function of human p100 protein are put forward.

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Available abstract

Objective: To construct eukaryotic green fluorescence protein expressing recombinant plasmids, pEGFP-C2-p100-SN and pEGFP-C2-p100-TD, which contained human p100 Staphylococcal Nuclease (SN) domain and Tudor (TD) domain respectively. Methods: The p100-SN and p100-TD fragments were purified after PSG5-p100-SN and PSG5-p100-TD were digested by Eco RI and Bam HI. And then they were inserted into pEGFP-C2 fluorescent expressing vector respectively. Results: The fragments of p100-SN and p100-TD were detected in the products of the restriction enzyme digestion and PCR. Conclusion: The fluorescent expressing recombinant plasmids, which expressed p100-SN-GFP and p100-TD-GFP fusion proteins, are constructed successfully. The studies about the function of human p100 protein are put forward.

Key concepts: Recombinant DNA, Green fluorescent protein, Plasmid, Molecular biology, Fusion protein, Biology, Transfection, Nuclease

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