Construction and expression of a pEGFP-C2-p100 recombinant plasmid

Objective: To construct eukaryotic green fluorescent protein(GFP) expressing recombinant plasmids, pEGFP-C2-p100, which contain human p100 full length cDNA. Methods: The p100 full length cDNA was purified after pSG5-p100 plasmids were digested by EcoR I and BamH I. And then they were inserted into pEGFP-C2 fluorescent expressing vector. We detected the p100 fragment by double digestion. This pEGFP-C2-p100 recombinant plasmids was transfected into HeLa cell line and the expression of green fluorescent protein was observed. Results:(1)The p100 fragment was detected in the products of the restriction enzyme digestion. (2)The green fluorescent protein could be observed by fluorescence microscope after the transfection. Conclusion:(1)The full length of p100 cDNA fragment is inserted into the pEGFP-C2 vector. (2)The fluorescent expressing recombinant plasmids pEGFP-C2-p100 could express p100-GFP fusion proteins successfully.