Construction and Expression of pERFP-C1-hTudor-SN-SN (1-4) Recombinant Eukaryotic Plasmids

Objective: To construct eukaryotic red fluorescent protein (RFP) expressing recombinant plasmids,pERFP-C1-hTudor-SN-SN(1-4),which contain SN((1-4) fragments of human Tudor-SN.Methods: The genes of Tudor-SN fragments were amplified by PCR from the recombinant pSG5-Tudor-SN-flag plasmid and inserted into pERFP-C1 fluorescent expressing vector with EcoRⅠ and BamHⅠ site.These recombinant pERFP-C1-hTudor-SN-SN (1-4) plasmids were transfected into HeLa cells and the expression of red fluorescent fusion proteins was examined by fluorescence microscope.Results: The SN(1-4) fragments of Tudor-SN were sequenced correctly and detected in the products of the restriction single/double enzyme digestion.The red fluorescent fusion proteins were observed in HeLa cell after the transfection.Conclusion: These recombinant eukaryotic plasmids of pERFP-C1-Tudor-SN(1-4) were constructed successfully and expressed effectively.