ScholarLens Back to search
2003•Zhongguo shouyi zazhiRequires access

Cloning and sequence analysis of gene encoding outer capsid protein VP7 of porcine rotavirus(RV) JL94 strain isolated in China

Shuhong Chen

Open publisher page 0 citations

Abstract

The native JL94 strain of porcine rotavirus was propagated and harvested on MA104 cell. Using a pair of primer designed according to the published cDNA sequence of RV's VP7 gene , the full length gene encoding outer capsid protein VP7 was amplified from JL94 strain by reverse transcription-polymerase chain reaction (RT-PCR). The cDNA of VP7gene was inserted into pMD18-T vector and was identified by sequencing, single and double digestion using HindⅢ, HindⅢ and BamHⅠ.The result of its homology comparison with C134/VP7, OSU/VP7, ICB2185/VP7 and YM/VP7 is 87%, 99%, 74% and 83% respectively.

About this research paper

What this paper is about

The native JL94 strain of porcine rotavirus was propagated and harvested on MA104 cell. Using a pair of primer designed according to the published cDNA sequence of RV's VP7 gene , the full length gene encoding outer capsid protein VP7 was amplified from JL94 strain by reverse transcription-polymerase chain reaction (RT-PCR). The cDNA of VP7gene was inserted into pMD18-T vector and was identified by sequencing, single and double digestion using HindⅢ, HindⅢ and BamHⅠ.The result of its homology comparison with C134/VP7, OSU/VP7, ICB2185/VP7 and YM/VP7 is 87%, 99%, 74% and 83% respectively.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The native JL94 strain of porcine rotavirus was propagated and harvested on MA104 cell. Using a pair of primer designed according to the published cDNA sequence of RV's VP7 gene , the full length gene encoding outer capsid protein VP7 was amplified from JL94 strain by reverse transcription-polymerase chain reaction (RT-PCR). The cDNA of VP7gene was inserted into pMD18-T vector and was identified by sequencing, single and double digestion using HindⅢ, HindⅢ and BamHⅠ.The result of its homology comparison with C134/VP7, OSU/VP7, ICB2185/VP7 and YM/VP7 is 87%, 99%, 74% and 83% respectively.

Key concepts: Capsid, Complementary DNA, Biology, Gene, Molecular biology, Cloning (programming), Rotavirus, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and sequence analysis of gene encoding outer capsid protein VP7 of porcine rotavirus(RV) JL94 strain isolated in China — Research Paper | ScholarLens