2010•Linchuang gandanbing zazhiRequires access
Inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene
Wang Li-jua
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Abstract
Objective To observe the inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene. Methods Four siRNAs against the X region of hepatitis B virus were chemically synthesized,and then were subjected to HepG2.2.15 cells by liposome-mediated transfection. The HBsAg and HbeAg in the supernatant were assayed by ELISA and HBV DNA by RT-PCR. Results At 30nmoL/L, the four siRNA had no obvious suppression in HBeAg and HbsAg expression (P0.05),while at the concentration of 60nmoL/L and 90nmoL/L,the inhibition of siRNA-1 and siRNA-4 were statistically significant with the inhibition rates of 41% and 43%;the HBV DNA replication was obviously reduced. Conclusion The chemically synthesized siRNAs can effectively inhibit hepatitis B viral replication in vitro.