Inhibition of hepatitis B surface antigen and hepatitis B e antigen expression by hairpin siRNAs targeted to hepatitis B virus S gene in HepG2215 cells in vitro

Objective To study the effect of hairpin siRNAs which expressed by constructed plasmid on hepatitis B surface antigen and hepatitis B e antigen expression in vitro.Methods Constructed plasmid pSilencer 2.1-U6-S that express hairpin siRNAs targeted to HBV S gene was co-transfected with ayw HBV expression plasmid into HepG2215 cells.ELISA and reverse transcript(RT)-PCR were used to detected the change of HBV 2.4 kb RNA,HBsAg and HBeAg in HepG2215 cells before and after treatment with siRNA.Results After treatment with siRNA,the level of(2.1)kb RNA was reduced by 75.2% and HBsAg and HBeAg were also reduced by 63.4% and 68.0%,respectively.Conclusion siRNA can effectively inhibit replication of HBV in vitro.