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[Specific small interfering RNAs inhibit replication of hepatitis B virus].

Jing Jiao, Hong Cao, Meijuan Zhou, Zhihua Liu, Zhenhua Ding

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Abstract

OBJECTIVE: To obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect. METHODS: Three siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection. RESULTS: The cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05). CONCLUSION: Specific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.

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OBJECTIVE: To obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect. METHODS: Three siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection. RESULTS: The cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05). CONCLUSION: Specific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.

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Available abstract

OBJECTIVE: To obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect. METHODS: Three siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection. RESULTS: The cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05). CONCLUSION: Specific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.

Key concepts: Small interfering RNA, Transfection, HBsAg, Hepatitis B virus, Molecular biology, Messenger RNA, RNA interference, Virology

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