Determination of amikacin with alizarin red by charge transfer spectrophotometry

The charge transfer reaction between Amikacin and alizarin red in water solution was investigated by spectrophotometry.At room temperature,Amikacin could react with alizarin red to form a 1:1 complex in the presence of water,and its maximum absorption wavelength is at 520 nm.The apparent molar absorptivity of complex is 1.44×103 L·mol-1·cm-1 at 520 nm.The Beer's Law is obeyed in the range of 5×10-5 mol/L~6×10-4 mol/L of Amikacin.The linear regression equation is A=0.23+1439.5c (mol/L) with a linear correlation coefficient 0f 0.9992 and the RSD of six determination is 0.31%.The recovery is 97.6 %~101.6 %.The proposed method with simple and rapid has been applied to the determination of Amikacin in sample with satisfactory results.