Imipenem resistance pseudomonas aeruginosa drug resistance analysis and a metal beta lactamase detection

Objective To survey hospital inpatients samples of Pseudomonas aeruginosa′s drug resistance to imipenem and the level of production of metal-β-lactamases(MBLs).Methods Isolated from clinical specimens of pseudomonas aeruginosa were used French bioMerieux bacterial identification and susceptibility testing system for the identification of bacteria and drug sensitivity test of the slip,collaborative experiment was used detect Pseudomonas aeruginosa producing metal-β-lactamase situation.Results 63 of 236 pseudomonas aeruginosa had imipenem resistance,resistance rate was 26.69%,imipenem-resistant pseudomonas aeruginosa for amikacin,gentamicin,piperacillin/tazobactam,cephalosporinspiperazine ketone/sulbactam,ciprofloxacin first part of the resistance,resistance rates were 45.12%,50.33%,59.74%,61.43%,62.12% and 63.16%.Ceftazidime,cefepime,piperacillin,aztreonam,meropenem resistance rates were higher.63 imipenem-resistant pseudomonas aeruginosa metallo-β-lactamase detection rate was 38.10%(24/63).Conclusion Metallo-β-lactamase production is one of the mechanisms for pseudomonas aeruginosa to carbapenem antibiotic resistance,the laboratory should strengthen the monitoring of enzyme production strains for clinical use of antibiotics.