Studies on Ultrafiltration of Ganoderma Fermentation Broth and Exopolysaccharides

Intracellular polysaccharides from Ganoderma submerged fermentation was extracted by ultrafiltration, concentration and ethanol precipitation.The ultrafiltration conditions were: membrane relative molecular mass,10000;operating pressure,0.2 MPa;and initial concentration,15.79 mg/mL.Compared with vacuum concentration, higher yield and content of intracellular polysaccharides could be obtained by ultrafiltration and concentration,and it could improve the quality of polysaccharides.Ganoderma intracellular polysaccharides exhibited obvious scavenging effects for hydroxyl free radicals(HFR),which bad positive correlation with the initial concentration,and the HFR scavenging ratio of polysaccharides prepared by ultrafiltration and concentration was better than that by vacuum concentration.