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2006•jOURNAL OF southwest University for NationalitiesRequires access

Determination of xanthophyl contents in chrysanthemum morifolium ramat with HPLC

Siman Liu

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Abstract

The approach is established to determine the xanthophyl contents in chrysanthemum morifolium ramat.The high-proficiency liquid colour is adopted as method,xanthophyl as index and Diamonsil~(TM)C18(4.6mm 150mm,5μm)as column;CH_(3)OH-H_(2)O(95.5:4.5)as mobile phase.The wavelength for determination is 444.5mm.Results: The average rate of recovery is 99.23% and RSD is 0.6%.Lineal relation is better when the sample is 0.0027-0.0189μg r = 0.9998.The determination approach which can be used to control the quality of chrysanthemum is convenient,stable and has good recurrence characteristic.

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What this paper is about

The approach is established to determine the xanthophyl contents in chrysanthemum morifolium ramat.The high-proficiency liquid colour is adopted as method,xanthophyl as index and Diamonsil~(TM)C18(4.6mm 150mm,5μm)as column;CH_(3)OH-H_(2)O(95.5:4.5)as mobile phase.The wavelength for determination is 444.5mm.Results: The average rate of recovery is 99.23% and RSD is 0.6%.Lineal relation is better when the sample is 0.0027-0.0189μg r = 0.9998.The determination approach which can be used to control the quality of chrysanthemum is convenient,stable and has good recurrence characteristic.

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Available abstract

The approach is established to determine the xanthophyl contents in chrysanthemum morifolium ramat.The high-proficiency liquid colour is adopted as method,xanthophyl as index and Diamonsil~(TM)C18(4.6mm 150mm,5μm)as column;CH_(3)OH-H_(2)O(95.5:4.5)as mobile phase.The wavelength for determination is 444.5mm.Results: The average rate of recovery is 99.23% and RSD is 0.6%.Lineal relation is better when the sample is 0.0027-0.0189μg r = 0.9998.The determination approach which can be used to control the quality of chrysanthemum is convenient,stable and has good recurrence characteristic.

Key concepts: Chrysanthemum morifolium, Chromatography, Chemistry, High-performance liquid chromatography, Recovery rate, Horticulture, Biology

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