Optimizate on Submerged Fermentation Conditions of Ganoderma lucidum by Corn Mash

Objective: In order to raise the yield of Ganoderma lucidum polysaccharide,submerged fermentation conditions of Ganoderma lucidum were optimizated.Methods: Using medium of corn mash as main component,single factor experiment and orthogonal experiment were conducted to study submerged fermentation conditions such as fermentation temperature and shaking table rotation rate etc.which affecte the yield of Ganoderma lucidum polysaccharide.Results: By range analysis and variance analysis,the optimum submerged fermentatio conditions were obtained as followed: the fermentation temperature 27℃,the shaking table rotation rate 170r/min,the medium initial pH 6.0,the fermentation time 144h.Conclusion: By optimizating submerged fermentation conditions,the yield of Ganoderma lucidum polysaccharide could be raised remarkably.Under the optimum fermentation conditions,the yield of Ganoderma lucidum polysaccharid increased from 1.851g/L to 2.439g/L,that had been raised 31.0% as compared with the original yield.