Simultaneous Quantification of Four Compounds in Sanchuan Paeonol Ointment by GC

Objective To establish an efficacious gas chromatography(GC) method for the determination of camphor,menthol,borneol and paeonol in Sanchuan paeonol ointment.Methods The GC method was applied and the separation was performed by means of temperature programming on ZB-WAX capillary column(30 m×0.25 mm×0.25 μm).The analysis was carried out by external standard method on FID detector with nitrogen as the carrier gas.Results Camphor,menthol,borneol and paeonol in the ointment had a good linear relationship within the specific range,and the average recovery was 99.82%,99.28%,98.72% and 97.35%,respectively.Conclusion The method is simple and accurate,with good reproducibility and sensitivity,therefore can be used as a quality control method for the quantitative determination of camphor,menthol,borneol and paeonol in Sanchuan paeonol ointment.