Comparison of two rat models of chronic nephrosis

Objective To investigate the differences between two different methods in construction of rat nephropathy model,thus to provide references for building the different renal failure models. Methods The rat nephropathy models were established by resection of 5 /6 of the kidney( Group A) and by unilateral nephrectomy combined repeated injecting adriamycin( Group B). The renal function indexes( BUN and Cr),serum albumin,C-reactive protein( CRP), malondialdehyde( MDA) and superoxide dismutase( SOD) were recorded,and pathological changes were observed for comparison. All of above of the two groups were compared with normal rats( Group N). Results All blood biochemical indices of the experimental animals in Group A and B were changed after modeling. At the 4th,8thand 12thweekends,the concentrations of BUN,Cr,CRP and MDA in both Group A and B were significantly increased compared with Group N( P 0. 05),while serum albumin and SOD were significantly reduced( P 0. 05). Moreover,the concentration of CRP and MDA in Group B were increased significantly more prominent than Group A at the 8thweekend( P 0. 01),while the reduction of SOD was also significantly more prominent( P 0. 05). Focal renal interstitial inflammation was revealed in both Group A and B,while it was significantly severer in Group B at the 8thweekend. Conclusion Earlier microinflammatory state,oxidative stress state and renal interstitial inflammation are founded in the unilateral nephrectomy combined repeated injecting adriamycin model than in 5 /6 of kidney resection model.