Cloning and Aequence Analysis of ANS Gene fromLitchi chinensis

Taking the peel and young leaves of Litechi chinensis ‘Nuomici’at three different stages as material,its ANS gene was cloned and analyzed.The results showed that the anthocyanidin synthase(ANS) was a key enzyme in the anthocyanin biosynthesis.AANSgene was cloned from litchi pericarp by homologous cloning method.The open reading frame was 1 074bp,and encoding 357amino acids.The 1 279bp length fragment was amplified from genome and analysis,which contained five introns,respectively,in 504～708bp.It was found that the gene encoding for the protein had close relationship with mountain grape(Vitis vinifera),cocoa(Theobroma cacao),citrus(Citrus sinensis),and other fruit trees through phylogenetic analysis.