2011•Redai zuowu xuebaoRequires access
Cloning and Expression Analysis of a Lectin Gene from Wuhe Litchi
Jiabao Wang
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Abstract
Partial cDNA(JF920723)and gDNA(JF920724)sequences of a lectin gene(LcLec)were cloned from Wuhe litchi(Licthi chinensis Sonn.cv.Wuhe)using PCR.Sequence analysis showed that the cDNA sequence had an open reading frame(ORF)with 468 bp in length,encoding a polypeptide of 155 amino acids.The deduced amino acid sequence of LcLec had the typical conserved domain structure as mannose-binding lectin.The gDNA sequence contained three introns,the length of each intron was 124 bp,108 bp and 119 bp,respectively.RT-PCR analysis showed that the expression of LcLec in pericarp increased at early stage of fruit development and then decreased till fruit maturation.During the postharvest storage,the expression of LcLec in pericarp increased together with the percarp browning index.