Performance Evaluation of LH 750 Automatic Hematology Analyzer

Objective To evaluate the performance of LH 750 hematology analyzer.Methods The indexes including precision,total reproducibility,carry-over,linearity and comparability were examined according to published evaluation standard.At the same time,the indexes of differential count(DC) were compared between those produced by the analyzer and manual methods.Results Precision,carry-over and total reproducibility in counting of leucocytes,erythrocytes,platelets and examination of hemoglobin on the analyzer were showed in the prescriptive range.The determined results for linearity-control were expected ranges.The results correlated well(r20.963) and bias were little(-2.094%~1.284%) with those obtained on the comparative instrument.DC:reproducibility was good for neutrophils,lymphocytes,monocytes,eosinophils and basophils.Comparison of the results by instrument with manual for normal samples in morphology,the correlation was better for neutrophils,lymphocytes and eosinophils(r2=0.946 9~0.966 1);good for monocytes(r2=0.801 3),not good for basophils(r2=0.518 0).Comparison of instrument flags with manual DC for 296 samples,false positive ratio,false negative ratio,accuracy,specificity and sensitivity were 15.93%,4.35%,86.78%,84.07% and 95.65% separately.Conclusion The analyzer can be used to test blood cell parameters accurately and reliably.Its main performance indices accords with the experimental requirements;the results are credible.It is necessary to check with microscopy for DC before reported when it is doubted.