STUDIES ON TRANSFERRING CHITINASE GENE INTO POTATO VARIETY NEA 303

Microtubers of NEA 303, an extra early variety, were used as explants,and chitinase gene was introduced into the variety by means of agrobacterium mediated transformation.Firstly,microtubers as receptors and plasmid pBch as donor were co cultured for 3 days in MS + NAA 1mg/+ZT 2mg/L, then were transferred to the same medium plus kanamycin 50 mg/L and cefotaxime 200 mg/L.When shoots were 1～2 cm in length, they were transferred to radication medium containing kanamycin 75 mg/L for rhizogenesis.Tests of PCR and PCR Southern hybridization were made on 4 transgenic plants and 3 of those were positive reaction.Transformation rate was 3 2% in this study.