INTRODUCTION OF CHITINASE GENE FROM KIDNEY BEAN INTO POTATO BY AGROBACTERIUM MEDIATED TRANSFORMATION

Microtubers of two leading potato cultivars Luyin 1 and Kexin 4 were used as exlants in the study.The chitinase gene from kidney bean was introduced into potato by Agrobacterium mediated transformation.After three day's coculture with Agrobacterium tumefaciens,the discs of in vitro grown microtubers were transferred onto selection medium containing 100mg/L kanamycin to induce buds.When the shoots reached 1 0～1 5cm high,they were transferred into liquid medium containing 100mg/L kanamycin to select by rooting.Through the two cycles of selection,the PCR reactions of all transformed plants were positive,which indicated that the selection system was reliable.The transformation frequency of the early cultivar Luyin 1 was obviously higher than that of the late cultivar Kexin 4,with the highest frequency of 41 0% and 3 85 plants per explants.