Methods of transformation of AcInv gene into potato plants mediated by Agrobacterium tumefaciens

AcInv antisense gene was transformed by the Agrobacterium tumefaciens, LBA 4404 in which the plasmid pBIRA was as the vector for the gene expression. The results showed that: The selected callus induction media for the two kinds of explants was: MS+NAA 0.5 mg/L + 6BA 2.25 mg/L+GA310 mg/L, regeneration media was: MS + 6BA 2.25 mg/L + GA3 10 mg/L, the selected media for rooting was:MS+NAA 0.1 mg/L. The selective pressure during the callus induction and regeneration was Kanamycin 100 mg/L, thes selected pressure during regeneration was Kanamycin 50 mg/L. In the optimum regeneration system, the optimum bacterium concentration in value of OD600 was 0.5, it had a best promoting effect on stem segments and the leaf of discs. the optimum time of pre-culture, infection and co-culture for leaf discs were 3 days, 12 mins and 3 days respectively; to stem segments, they were 2 days, 8 mins and 2 days. Nine cultivars were chosen for transformation. In the transformation of potato using AcInv Antisense gene, transgenic plants were obtained from the callus derived from the stem segments of Atlantic cultivar. Using PCR methods assay showed that the regenerated plants had postive reaction, thus it could be proved that they are transformed plants, the primary detection expressed that AcInv antisense gene was transformed into potato genome.