Preparation and evaluation in vitro of terbinafine hydrochloride binary ethosomes

Objective To prepare and evaluate in vitro terbinafine hydrochloride binary ethosomes. Methods Terbinafine hydrochloride binary ethosomes were prepared by ethanol injection method. The formation was optimized by orthogonal design with particle size,Zeta potential and entrapment efficiency as indexes. The percutaneous permeation of different kinds of terbinafine hydrochloride liposomes were determined by Franz diffusion cells. Results Optimal formation was as follows: the mass fraction of drug was 0.5‰; the ratio of drug to lecithin was 1 ∶5( g ∶ g); the volume fraction of diol phase was 30%( ethanol: propylene glycol = 7∶3). The mean particle size,Zeta potential,and encapsulation efficiency were( 36.2± 1.0) nm,(-23. 22 ±2. 02) m V,and( 97.61± 0.09) %,respectively. The maximum in vitro epidermal-dermal retention amount at24 h was( 23. 18± 2.38) μg / cm2. Conclusion The optimized formulation was reasonable,and it could be used for the preparation of terbinafine hydrochloride binary ethosomes,which significantly improved the epidermal-dermal retention.