Preparation and Pharmaceutical Property of Prednisolone Acetate Ethosomes

OBJECTIVE:To prepare Prednisolone acetate ethosomes,and to evaluate their pharmaceutical property.METHODS:Prednisolone acetate loaded ethosomes were prepared with ethanol injection method.The formulation of ethosomes was optimized by orthogonal experiment with weight ratio of drug to soybean lecithin(A),weight ratio of cholesterol to soybean lecithin(B),ethanol concentration(C) as factors and using encapsulation efficiency as index.The morphology,particle size,Zeta potential,entrapment efficiency and stability of prepared ethosomes were determined.The thermal behaviors were investigated by differential scanning calorimetry(DSC).RESULTS:The optimal formulation was as follows:A was 1:20,B was 1:6,and C was 30%.The obtained ethosomes were spherical,the mean size and Zeta potential were(278.5±46.7) nm and(-31.6±0.04) mV,respectively.The entrapment efficiency of prednisolone acetate in ethosomes was(76.79±0.29) %.The ethosomes were stable within 30 days.DSC study showed that the prednisolone acetate encapsulated in ethosomes was in the amorphous form.CONCLUSION:The preparation technology is simple and convenient,and optimal formulation is up to the requirements of pharmaceutical property.