EFFECTS OF DIMETHYL SULFOXIDE AND SECOND-MESSENGER EFFECTOR ON CRYOPRESERVATION OF PLATELET ULTRASTRUCTURE

Objective To observe the effect of cryopreservation with 0.02 dimethyl sulfoxide(DMSO) and ThromboSol, a mixture of second-messenger effectors, on platelet ultrastructure. Methods The inspissated platelets were treated with 0.02 DMSO, 0.06 DMSO and 0.02 DMSO plus ThromboSol, respectively, and then placed in liquid nitrogen. The samples were thawed and the ultrastructure was observed by SEM and TEM one month and six months later. Results The platelets cryopreserved by 0.02 DMSO and ThromboSol retained good ultrastructure and that some platelets were in active status. There was no significant difference between one-month group and six-month group. Conclusion 0.02 DMSO and ThromboSol might effectively protect the platelet ultrastructure and the long-term cryopreservative effect is stable.