Effects of 2,4-D and 6-BA on Callus Initiation and Plantlet Regeneration from Mature Embryos of Indica Rice

[Objective] The study aimed to explore the effects of 2,4-D and 6-BA on indica rice callus culture using mature embryos as explants.[Method] 2,4-D and 6-BA were set at different concentrations in callus initiation media and plantlet regeneration media in order to study their effects on callus initiation,plantlet regeneration and root initiation.[Result] The calli of seven varieties,including Jiayu 948,Yanghui 559,Yangxian 6547,Zhongerruanzhan,Minghui 86,Guanghui 998,and Zunxian 3,were best initiated on callus initiation media containing 2,4-D at 0.5 mg/L;0.2 mg/L 6-BA was supplemented into callus initiation media containing optimal 2,4-D concentration with an objective to explore the effects of 6-BA on callus initiation and plantlet regeneration,and it was found that the 6-BA treatment had no significant effects on callus initiation frequency,but could reduce plantlet regeneration frequency;not only plantlet regeneration frequency could be maintained even increased,but also the quality of regenerated plantlets could be improved when the concentration of 6-BA in plantlet regeneration medium was properly reduced.[Conclusion] The results of this study could provide references for reasonable uses of 2,4-D and 6-BA in indica rice callus culture.