The Pathogenesis of Aplastic Anemia and the Influence of Cyclosporin A on Aplastic Anemia in Vitro

Objective:To explore the pathogenesis of aplastic anemia (AA) and the influence of cyclosporin A (CsA) on bone marrow (BM) cells from AA patients in vitro.Methods:Eighteen AA patients were tested to observe the BM CFU GM colony formation and the influence of AA BM cells and sera on normal BM CFU GM growth by using methylcellulose micromethod.According to this the pathogenesis of AA was classified.At the same time CsA was cocultured with AA and normal BM.Results:There were 5 cases with stem cells defect,7 cases with cells suppression,2 cases with sera suppression and 4 cases with microenvironmental defect.The BM CFU GM colony formation of AA was significantly lower than that of normal control ( P 0.01).CsA could increase the colony and cluster formation of AA group ( P 0.05).8 cases of AA BM were sensitive to CsA.Among them there were 6 cases with cell suppression and 2 cases with stem cells defect.Conclusion:This study would provide experimental basis for predicting clinical curative effect of CsA on AA.