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Isolation and Identification of a Low Rirulent Newcastle disease virus Strain in Pigeon

Jun Ling Lu

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Abstract

6 pigeons from a pigeon farm in Shanghai were diagnosed by laboratory methods,including: clinical diagnosis;bacterial isolation;NDV fluorescence RT-PCR detection;SPF chicken embryo isolation and identification.The HA and HI tests indicated that the isolated strain was NDV.The 50% embryo infectious dose(EID50) and intracerabral pathogenicity index(ICPI) of SPF chicken embryo allantoid liquid were 107.63 and 1.19,respectively.According to the clinical symptom,EID50,ICPI,the isolated pigeon NDV is a low virulent strain.

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What this paper is about

6 pigeons from a pigeon farm in Shanghai were diagnosed by laboratory methods,including: clinical diagnosis;bacterial isolation;NDV fluorescence RT-PCR detection;SPF chicken embryo isolation and identification.The HA and HI tests indicated that the isolated strain was NDV.The 50% embryo infectious dose(EID50) and intracerabral pathogenicity index(ICPI) of SPF chicken embryo allantoid liquid were 107.63 and 1.19,respectively.According to the clinical symptom,EID50,ICPI,the isolated pigeon NDV is a low virulent strain.

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Available abstract

6 pigeons from a pigeon farm in Shanghai were diagnosed by laboratory methods,including: clinical diagnosis;bacterial isolation;NDV fluorescence RT-PCR detection;SPF chicken embryo isolation and identification.The HA and HI tests indicated that the isolated strain was NDV.The 50% embryo infectious dose(EID50) and intracerabral pathogenicity index(ICPI) of SPF chicken embryo allantoid liquid were 107.63 and 1.19,respectively.According to the clinical symptom,EID50,ICPI,the isolated pigeon NDV is a low virulent strain.

Key concepts: Newcastle disease, Virulence, Isolation (microbiology), Virology, Biology, Virus isolation, Embryo, Strain (injury)

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