Construction of Bone Mesenchymal Stem Cells(bMSCs) Transfected with bFGF in Vitro and the Enhancement of bMSCs

Objective: To construct a retroviral vector carrying both enhanced green fluorescent protein(EGFP) and human basic fibroblast growth factor(bFGF) gene for observing the enhanced effect of transfected bone mesenchymal stem cells(bMSCs) and gene expression.Methods: bMSCs were constructed from bones of rabbits.The pIRES2-EGFP-bFGF-pcDNA3 expression plasmid was transfected into bMSCs with liposome lipofectamine.The expression of EGFP was observed by inverted fluorescence microscope and was calculated by transfection efficiency.The proliferativity of the transfected cells was detemined with MTT assay.Results: The recombinant retroviral vector bFGF-pcDNA3 and pIRES2-EGFP was successfully constructed.The bMSCs infected by both vectors stably gave out green fluorescence under microscope.The transfection efficiency was(43.32±4.51)%.Transfected cells grew more quiclkly than that of non-transfected cells(P0.05).Conclusion: Rabbit bMSCs can be successfully transfected by using pIRES2-EGFP-bFGF-pcDNA3 plasmid.This vector can be easily traced.