Biological characteristics of mesenchymal stem cells transfected with EGFP gene mediated by retroviral vector

Objective Stably introduce enhanced green fluorescent protein gene(EGFP) to bone marrow mesenchymal stem cells(BMSCs) and investigate the change of biological characteristics.To approach the feasibility of EGFP be applied as a stable morphological marker for BMSCs in vivo.Methods Packaging cells PT67 were transfected by pLEGFP-N1 retroviral vector,and BMSCs were transduced by viral supernatant infection.BMSCs stably express EGFP(EGFP-BMSCs) was obtained by be selected with G418 and single cell cloning.EGFP-BMSCs was compared with BMSCs in growth curve,cytoactive and adherence rate.Results EGFP was stably introduced to BMSCs by retroviral vector.The biological characteristics of EGFP-BMSCs were consistent with those of BMSCs and fluorescent light expression can persist three months.Conclusions EGFP can be applied as a stable morphological marker for BMSCs in vivo.