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2010•Journal of Clinical StomatologyRequires access

Construction of eukaryotic expression vectors of basic fibroblast growth factor and transfection in rat bone marrow stromal cells

Xie Long-chua

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Abstract

Objective:To investigate approach and possibility of transferring basic fibroblast growth factor(bFGF) gene into rat bone marrow stromal cells(BMSCs).Method:The eukaryotic expression vectors harboring bFGF cDNA were constructed and transfected into rat BMSCs mediated by liposome.The transcription and expression of bFGF gene in the transfected BMSCs were detected by means of immunohistochemistry,RT-PCR and western blot.Result:Stable overexpression of bFGF protein was detected in the transfected BMSCs.Conclusion:bFGF gene can be transfected into BMSCs and stably express in transfected BMSCs.

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What this paper is about

Objective:To investigate approach and possibility of transferring basic fibroblast growth factor(bFGF) gene into rat bone marrow stromal cells(BMSCs).Method:The eukaryotic expression vectors harboring bFGF cDNA were constructed and transfected into rat BMSCs mediated by liposome.The transcription and expression of bFGF gene in the transfected BMSCs were detected by means of immunohistochemistry,RT-PCR and western blot.Result:Stable overexpression of bFGF protein was detected in the transfected BMSCs.Conclusion:bFGF gene can be transfected into BMSCs and stably express in transfected BMSCs.

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Available abstract

Objective:To investigate approach and possibility of transferring basic fibroblast growth factor(bFGF) gene into rat bone marrow stromal cells(BMSCs).Method:The eukaryotic expression vectors harboring bFGF cDNA were constructed and transfected into rat BMSCs mediated by liposome.The transcription and expression of bFGF gene in the transfected BMSCs were detected by means of immunohistochemistry,RT-PCR and western blot.Result:Stable overexpression of bFGF protein was detected in the transfected BMSCs.Conclusion:bFGF gene can be transfected into BMSCs and stably express in transfected BMSCs.

Key concepts: Transfection, Basic fibroblast growth factor, Stromal cell, Molecular biology, Bone marrow, Complementary DNA, Western blot, Biology

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