The application of cDNA microarrays technology in mechanism of drug

Objective To study the application of cDNA microarrays in the mechanism analysis for drug.Methods Rat model bearing NuTu-19 cells was made and the changes of biology phenotype in tumor cells were observed after treatment with Basil polysaccharide for 50 days.Total RNA of the tumor tissue was extracted,and the fluorescent cDNA probes were prepared through reverse transcription of the isolated mRNAs.The RNA samples were labeled with Cy3-dUTP or Cy5-dUTP,then hybridized with the microarrays.The chips were then scanned with a ScanArray.The acquired images were analyzed using ImaGene 3.0 software.Results Compared with the controls,168 genes showed different expressions.Sixty-five genes showed more than three times of expression difference,all of which were downregulated.One hundred and three genes showed twice of expression difference,in which 43 genes were upregulated while 60 genes were downregulated.Conclusions cDNA microarray technology may be a powerful tool in discovering the possible mechanism of drug in gene level,provide new informations,and accelerates the development of new drugs.